New to MyHealth?
Manage Your Care From Anywhere.
Access your health information from any device with MyHealth. You can message your clinic, view lab results, schedule an appointment, and pay your bill.
ALREADY HAVE AN ACCESS CODE?
DON'T HAVE AN ACCESS CODE?
NEED MORE DETAILS?
MyHealth for Mobile
Reporter gene imaging of protein-protein interactions in living subjects
Reporter gene imaging of protein-protein interactions in living subjects CURRENT OPINION IN BIOTECHNOLOGY Massoud, T. F., Paulmurugan, R., De, A., Ray, P., Garnbhir, S. S. 2007; 18 (1): 31-37Abstract
In the past few years there has been a veritable explosion in the field of reporter gene imaging, with the aim of determining the location, duration and extent of gene expression within living subjects. An important application of this approach is the molecular imaging of interacting protein partners, which could pave the way to functional proteomics in living animals and might provide a tool for the whole-body evaluation of new pharmaceuticals targeted to modulate protein-protein interactions. Three general methods are currently available for imaging protein-protein interactions in living subjects using reporter genes: a modified mammalian two-hybrid system, a bioluminescence resonance energy transfer (BRET) system, and split reporter protein complementation and reconstitution strategies. In the future, these innovative approaches are likely to enhance our appreciation of entire biological pathway systems and their pharmacological regulation.
View details for DOI 10.1016/j.copbio.2007.01.007
View details for Web of Science ID 000244593000006
View details for PubMedID 17254764